Overproduction and immuno-affinity purification of myelin proteolipid protein (PLP), an inositol hexakisphosphate-binding protein, in a baculovirus expression system.

Fukuzono, S; Takeshita, T; Sakamoto, T; Hisada, A; Shimizu, N; Mikoshiba, K

Fukuzono, S; Takeshita, T; Sakamoto, T; Hisada, A; Shimizu, N; Mikoshiba, K.

Affiliation

Fukuzono S; Advanced Research Laboratory, Hitachi Ltd., Saitama, Japan. fukuzono@cm.naka.hitachi.co.jp

Biochem Biophys Res Commun ; 249(1): 66-72, 1998 Aug 10.

Article in En | MEDLINE | ID: mdl-9705833

Subject(s)

Baculoviridae/metabolism; Myelin Proteolipid Protein/biosynthesis; Myelin Proteolipid Protein/isolation & purification; Aged; Animals; Baculoviridae/genetics; Chromatography, Affinity; Humans; Immunosorbent Techniques; Mice; Myelin Proteolipid Protein/genetics; Myelin Proteolipid Protein/immunology; Phytic Acid/metabolism; Protein Binding; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Recombinant Proteins/isolation & purification

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Collection: 01-internacional Database: MEDLINE Main subject: Baculoviridae / Myelin Proteolipid Protein Limits: Aged / Animals / Humans Language: En Journal: Biochem Biophys Res Commun Year: 1998 Document type: Article Affiliation country: Japan

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