Glycerol diversifies phage repertoire selections and lowers non-specific phage absorption.
FEBS Lett
; 431(3): 448-52, 1998 Jul 24.
Article
in En
| MEDLINE
| ID: mdl-9714561
ABSTRACT
Using a semi-synthetic phage displayed antibody repertoire, isoform-specific and cross-reactive phage-antibodies to eukaryotic elongation factor 1A (eEF1A) have been selected. Enrichment of specific antibodies was found to depend on the presence of glycerol. Further selections against lactate dehydrogenase (LDH) revealed that the dominance of a phage-antibody clone to LDH was inhibited by glycerol, a notable feature for selection strategies where a broad variety of binding clones is desired. The impact of glycerol in distinct steps of the selection protocol was examined and glycerol found to affect certain antibody-antigen interactions. Furthermore, the nonspecific phage binding was lowered by three orders of magnitude at a 20% (v/v) glycerol concentration.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coliphages
/
Glycerol
/
Antibodies
Language:
En
Journal:
FEBS Lett
Year:
1998
Document type:
Article
Affiliation country:
Denmark
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM