A rare case of false positive in a yeast two-hybrid screening: the selection of rearranged bait constructs that produce a functional gal4 activity.

El Housni, H; Vandenbroere, I; Perez-Morga, D; Christophe, D; Pirson, I

El Housni, H; Vandenbroere, I; Perez-Morga, D; Christophe, D; Pirson, I.

Affiliation

El Housni H; Facultéde Médecine, UniversitéLibre de Bruxelles, 808 route de Lennik, Bât. C, Brussels, 1070, Belgium.

Anal Biochem ; 262(1): 94-6, 1998 Aug 15.

Article in En | MEDLINE | ID: mdl-9735157

Subject(s)

Calcium-Binding Proteins/genetics; Fungal Proteins/genetics; Saccharomyces cerevisiae Proteins; Transcription Factors; Amino Acid Sequence; Animals; Base Sequence; DNA-Binding Proteins; Dogs; Genes, Reporter/genetics; Molecular Sequence Data; Plasmids/genetics; Promoter Regions, Genetic/genetics; Saccharomyces cerevisiae/metabolism; Sequence Deletion/genetics; Transcriptional Activation/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Calcium-Binding Proteins / Fungal Proteins / Saccharomyces cerevisiae Proteins Type of study: Diagnostic_studies / Screening_studies Limits: Animals Language: En Journal: Anal Biochem Year: 1998 Document type: Article Affiliation country: Belgium

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