Carbamoyl-phosphate synthetase II in kinetoplastids.
Biochim Biophys Acta
; 1387(1-2): 462-8, 1998 Sep 08.
Article
in En
| MEDLINE
| ID: mdl-9748664
ABSTRACT
Genes for carbamoyl-phosphate synthetase II (CPS II), the first enzyme of de novo pyrimidine biosynthesis, were cloned from kinetoplastids, Trypanosoma cruzi and Leishmania mexicana. T. cruzi CPS II gene encodes a protein of 1524 amino acids that encompasses the glutaminase and CPS domains, but incorporates neither aspartate carbamoyltransferase nor dihydroorotase. The residue corresponding to lysine 993 of Escherichia coli CPS, a residue that characterizes the CPS inhibited by UMP and that is replaced by tryptophan in those inhibited by UTP, is in kinetoplastids a hydrophilic glutamine, in line with the preferential inhibition by UDP of kinetoplastid CPS II.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosoma cruzi
/
Leishmania mexicana
/
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Limits:
Animals
Language:
En
Journal:
Biochim Biophys Acta
Year:
1998
Document type:
Article
Affiliation country:
Japan