The interleukin 2 receptor alpha chain/CD25 promoter is a target for nuclear factor of activated T cells.
J Exp Med
; 188(7): 1369-73, 1998 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-9763616
ABSTRACT
The expression of the murine interleukin (IL)-2 receptor alpha chain/CD25 is strongly induced at the transcriptional level after T cell activation. We show here that nuclear factor of activated T cell (NF-AT) factors are involved in the control of CD25 promoter induction in T cells. NF-ATp and NF-ATc bind to two sites around positions -585 and -650 located upstream of the proximal CD25 promoter. Immediately 3' from these NF-AT motifs, nonconsensus sites are located for the binding of AP-1-like factors. Mutations of sites that suppress NF-AT binding impair the induction and strong NF-ATp-mediated transactivation of the CD25 promoter in T cells. In T lymphocytes from NF-ATp-deficient mice, the expression of CD25 is severely impaired, leading to a delayed IL-2 receptor expression after T cell receptor (TCR)/CD3 stimulation. Our data indicate an important role for NF-AT in the faithful expression of high affinity IL-2 receptors and a close link between the TCR-mediated induction of IL-2 and IL-2 receptor alpha chain promoters, both of which are regulated by NF-AT factors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Nuclear Proteins
/
Lymphocyte Activation
/
T-Lymphocytes
/
Receptors, Interleukin-2
/
Promoter Regions, Genetic
/
DNA-Binding Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
J Exp Med
Year:
1998
Document type:
Article
Affiliation country:
Germany