[Vimentin and Nup 180 in vitro binding assay].
Shi Yan Sheng Wu Xue Bao
; 29(4): 357-63, 1996 Dec.
Article
in Zh
| MEDLINE
| ID: mdl-9772680
ABSTRACT
In order to investigate relationship between vimentin and nuclear pore complex, we examined binding ability of vimentin, expressed in E. coli, with nucleoporin, isolated from rat liver nuclei, in vitro. Negative staining electron microscopy showed that the vimentin expressed in bacteria assembled 10 nm filament in vitro. SDS-PAGE and western blotting showed that Nup 180 bind to vimentin in binding assay in vitro. Combining immunogold labeling and negative staining electron microscopy techniques, we showed that Nup 180 bind on the 10 nm vimentin filaments. The experiment results indicated that vimentin filament may be anchored on nuclear pore complex in vivo by binding with Nup 180.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vimentin
/
Nuclear Proteins
/
Nuclear Pore Complex Proteins
Limits:
Animals
Language:
Zh
Journal:
Shi Yan Sheng Wu Xue Bao
Year:
1996
Document type:
Article