Evidence for zanamivir resistance in an immunocompromised child infected with influenza B virus.
J Infect Dis
; 178(5): 1257-62, 1998 Nov.
Article
in En
| MEDLINE
| ID: mdl-9780244
ABSTRACT
Zanamivir, a neuraminidase inhibitor, has shown promise as a drug to control influenza. During prolonged treatment with zanamivir, a mutant virus was isolated from an immunocompromised child infected with influenza B virus. A hemagglutinin mutation (198 Thr-->Ile) reduced the virus affinity for receptors found on susceptible human cells. A mutation in the neuraminidase active site (152 Arg-->Lys) led to a 1000-fold reduction in the enzyme sensitivity to zanamivir. When tested in ferrets, the mutant virus had less virulence than the parent; however, it had a growth preference over the parent in zanamivir-treated animals. Despite these changes, the sensitivity of the mutant virus to zanamivir assessed by a standard test in MDCK cells was unaffected. These data indicate that the current methods for monitoring resistant mutants are potentially flawed because no tissue culture system adequately reflects the receptor specificity of human respiratory tract epithelium.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Influenza B virus
/
Receptors, Virus
/
Sialic Acids
/
Immunosuppression Therapy
/
Orthomyxoviridae Infections
/
Influenza, Human
/
Mutation
Limits:
Animals
/
Female
/
Humans
/
Infant
Language:
En
Journal:
J Infect Dis
Year:
1998
Document type:
Article
Affiliation country:
United States