An acquired sensitivity to H2O2-induced apoptosis during neuronal differentiation of NT2/D1 cells.
Neuroreport
; 9(14): 3207-11, 1998 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-9831452
ABSTRACT
Brief exposure of neuronally differentiated human NT2/D1 cells to hydrogen peroxide induced cell death by apoptosis with an ED50 of 30 microM, whereas a 70-fold higher concentration was required to obtain an ED50 effect in undifferentiated NT2/D1 neuronal precursor cells. This enhanced sensitivity in NT2/D1 neurons was correlated with an 8-fold lower level of intracellular glutathione. Pretreatment with N-acetyl-L-cysteine, an agent that is able to raise the levels of intracellular glutathione, promoted the survival of hydrogen peroxide-treated NT2/D1 neurons. Thus, the low glutathione level may contribute to the high sensitivity of NT2/D1 neurones to hydrogen peroxide-induced apoptosis. This study indicates that neuronal susceptibility to oxidative damage is developmentally regulated.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxidants
/
Apoptosis
/
Hydrogen Peroxide
/
Neurons
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Neuroreport
Journal subject:
NEUROLOGIA
Year:
1998
Document type:
Article
Affiliation country:
Sweden