Surface investigations on the development of a direct optical immunosensor.
Biosens Bioelectron
; 13(10): 1141-7, 1998 Nov 01.
Article
in En
| MEDLINE
| ID: mdl-9842709
ABSTRACT
In the present paper surface studies for the development of a direct optical immunosensor for fast diagnosis of a myocardial infarction are presented. A fatty acid binding protein was detected by monoclonal antibodies. The applied measuring system was the grating coupler BIOS-1. Based on commercially available transducer materials protein immobilisation techniques have been developed and characterised by TOF-SIMS, AFM and EM. Three different label-free assay types were investigated. Only one assay leads to a sensitive and regenerable sensor set-up. It was possible to detect concentrations of the fatty acid binding protein down to 330 ng/ml. The general applicability of a direct optical immunosensor in the field of myocardial infarction diagnosis was demonstrated by this.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoassay
/
Tumor Suppressor Proteins
/
Optics and Photonics
/
Myocardial Infarction
/
Neoplasm Proteins
Limits:
Humans
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
1998
Document type:
Article
Affiliation country:
Germany