Positional cloning and molecular characterization of an immunodominant cytotoxic determinant of the mouse H3 minor histocompatibility complex.
Immunity
; 9(5): 687-98, 1998 Nov.
Article
in En
| MEDLINE
| ID: mdl-9846490
ABSTRACT
Immune responses to minor histocompatibility antigens are poorly understood and present substantial barriers to successful solid tissue and bone marrow transplantation among MHC-matched individuals. We exploited a unique positional cloning approach relying on the potent negative selection capability of cytotoxic T cells to identify the H3a gene responsible for immunodominant H2-Db-restricted determinants of the classically defined mouse autosomal H3 complex. The allelic basis for reciprocal H3a antigens is two amino acid changes within a single nonamer H2-Db-binding peptide. The H3a gene, now called Zfp106, encodes a 1888-amino acid protein with three zinc fingers and a beta-transducin domain consistent with DNA/protein binding. A region of ZFP106 is identical to a 600-amino acid sequence implicated in the insulin receptor signaling pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Minor Histocompatibility Antigens
/
Immunodominant Epitopes
/
Adaptor Proteins, Signal Transducing
/
Minor Histocompatibility Loci
/
Muscle Proteins
Limits:
Animals
Language:
En
Journal:
Immunity
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
1998
Document type:
Article
Affiliation country:
United States