Comet assay responses as indicators of carcinogen exposure.
Mutagenesis
; 13(6): 539-55, 1998 Nov.
Article
in En
| MEDLINE
| ID: mdl-9862185
ABSTRACT
Over 200 agents/factors have been examined in the single cell gel electrophoresis assay, more commonly known as the Comet assay, performed either in vitro or in vivo in a variety of species. Unequivocal carcinogenicity data are available for 119 of them, amongst which unequivocal Comet assay data exist for 95 agents. Of these 95 agents the prevalence of carcinogens was 88% (84/95). The carcinogens that were Comet positive (sensitivity) formed 88% (74/84), the non-carcinogens that were Comet negative (specificity) formed 64% (7/11). This simple analysis of the Comet assay has not taken account of the difference between in vitro and in vivo responses, species differences or organ and tissue differences. Also, limitations as to the conduct of the assay have not been examined in any depth. Thus, at the present time the Comet assay has high sensitivity for carcinogens, but its specificity is uncertain because few non-carcinogens have been tested.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinogens
/
Carcinogenicity Tests
/
Electrophoresis
/
Environmental Exposure
/
Mutagenicity Tests
Type of study:
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Mutagenesis
Journal subject:
GENETICA MEDICA
/
SAUDE AMBIENTAL
Year:
1998
Document type:
Article
Affiliation country:
United kingdom