An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members.
FEBS Lett
; 440(1-2): 119-24, 1998 Nov 27.
Article
in En
| MEDLINE
| ID: mdl-9862439
ABSTRACT
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Neoplastic
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Promoter Regions, Genetic
/
Proto-Oncogene Proteins c-jun
/
Proto-Oncogene Proteins c-fos
/
Transcription Factor AP-1
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
1998
Document type:
Article
Affiliation country:
Italy