Mutations of Gly to Ala in human glutathione transferase P1-1 affect helix 2 (G-site) and induce positive cooperativity in the binding of glutathione.

Lo Bello, M; Nuccetelli, M; Chiessi, E; Lahm, A; Mazzetti, A P; Battistoni, A; Caccuri, A M; Oakley, A J; Parker, M W; Tramontano, A; Federici, G; Ricci, G

Lo Bello, M; Nuccetelli, M; Chiessi, E; Lahm, A; Mazzetti, A P; Battistoni, A; Caccuri, A M; Oakley, A J; Parker, M W; Tramontano, A; Federici, G; Ricci, G.

Affiliation

Lo Bello M; Department of Biology, University of Rome "Tor Vergata", Italy. Lobello@uniroma2.it

J Mol Biol ; 284(5): 1717-25, 1998 Dec 18.

Article in En | MEDLINE | ID: mdl-9878382

Subject(s)

Glutathione Transferase/chemistry; Glutathione Transferase/genetics; Glutathione/metabolism; Isoenzymes/chemistry; Isoenzymes/genetics; Mutation; Alanine; Binding Sites; Circular Dichroism; Cysteine/chemistry; Escherichia coli/genetics; Glutathione S-Transferase pi; Glutathione Transferase/metabolism; Glycine; Humans; Isoenzymes/metabolism; Kinetics; Models, Molecular; Protein Conformation; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spectrophotometry, Ultraviolet; Tryptophan/chemistry

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Collection: 01-internacional Database: MEDLINE Main subject: Glutathione / Glutathione Transferase / Isoenzymes / Mutation Limits: Humans Language: En Journal: J Mol Biol Year: 1998 Document type: Article Affiliation country: Italy Country of publication: Netherlands

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