Large-scale preparation of biologically active recombinant chicken obese protein (leptin).

Raver, N; Taouis, M; Dridi, S; Derouet, M; Simon, J; Robinzon, B; Djiane, J; Gertler, A

Raver, N; Taouis, M; Dridi, S; Derouet, M; Simon, J; Robinzon, B; Djiane, J; Gertler, A.

Affiliation

Raver N; Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot, 76100, Israel.

Protein Expr Purif ; 14(3): 403-8, 1998 Dec.

Article in En | MEDLINE | ID: mdl-9882575

Subject(s)

Chickens/metabolism; Proteins/isolation & purification; Receptors, Cell Surface; Amino Acid Sequence; Animals; Appetite/drug effects; B-Lymphocytes/drug effects; Carrier Proteins/drug effects; Carrier Proteins/genetics; Cell Line; Chromatography, Liquid; DNA, Complementary/genetics; Depression, Chemical; Escherichia coli/genetics; Feeding Behavior/drug effects; Humans; Inclusion Bodies/chemistry; Leptin; Male; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Protein Biosynthesis; Protein Folding; Proteins/administration & dosage; Proteins/genetics; Proteins/pharmacology; Receptors, Leptin; Recombinant Fusion Proteins/administration & dosage; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/pharmacology; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Chickens / Receptors, Cell Surface Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 1998 Document type: Article Affiliation country: Israel

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