Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain ac10: gene cloning and enzyme purification and characterization.

Kulakova, L; Galkin, A; Kurihara, T; Yoshimura, T; Esaki, N

Kulakova, L; Galkin, A; Kurihara, T; Yoshimura, T; Esaki, N.

Affiliation

Kulakova L; Institute for Chemical Research, Kyoto University, Uji, Kyoto-Fu 611, Japan.

Appl Environ Microbiol ; 65(2): 611-7, 1999 Feb.

Article in En | MEDLINE | ID: mdl-9925590

Subject(s)

Gram-Negative Facultatively Anaerobic Rods/enzymology; Serine Endopeptidases/isolation & purification; Serine Endopeptidases/metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Ribosomal/chemistry; DNA, Ribosomal/genetics; Escherichia coli/genetics; Gram-Negative Facultatively Anaerobic Rods/genetics; Hydrogen-Ion Concentration; Molecular Sequence Data; RNA, Ribosomal, 16S/genetics; Recombinant Proteins; Sequence Analysis, DNA; Serine Endopeptidases/genetics; Substrate Specificity; Temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serine Endopeptidases / Gram-Negative Facultatively Anaerobic Rods Language: En Journal: Appl Environ Microbiol Year: 1999 Document type: Article Affiliation country: Japan Country of publication: United States

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