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Landscape of infection enhancing antibodies in COVID-19 and healthy donors
Hendra S Ismanto; Zichang Xu; Dianita S Saputri; Jan Wilamowski; Songling Li; Dendi K Nugraha; Yasuhiko Horiguchi; Masato Okada; Hisashi Arase; Daron M Standley.
Affiliation
  • Hendra S Ismanto; Osaka University
  • Zichang Xu; Osaka University
  • Dianita S Saputri; Osaka University
  • Jan Wilamowski; Osaka University
  • Songling Li; Osaka University
  • Dendi K Nugraha; Osaka University
  • Yasuhiko Horiguchi; Osaka University
  • Masato Okada; Osaka University
  • Hisashi Arase; Osaka University
  • Daron M Standley; Osaka University
Preprint in En | PREPRINT-BIORXIV | ID: ppbiorxiv-499414
ABSTRACT
To assess the frequency of SARS-CoV-2 infection enhancing antibodies in the general population, we searched over 64 million heavy chain antibody sequences from healthy and COVID-19 patient repertoires for sequences similar to 11 previously reported enhancing antibodies. Although the distribution of sequence identities was similar in COVID-19 and healthy repertoires, the COVID-19 hits were significantly more clonally expanded than healthy hits. Furthermore, among the tested hits, 17 out of 94 from COVID-19, compared with 2 out of 96 from healthy, bound to the enhancing epitope. A total of 6 of the 19 epitope-binding antibodies enhanced ACE2 receptor binding to the spike protein. Together, this study revealed that enhancing antibodies are far more frequent in COVID-19 patients than in healthy donors, but a reservoir of potential enhancing antibodies exists in healthy donors that could potentially mature to actual enhancing antibodies upon infection.
License
cc_by_nc
Full text: 1 Collection: 09-preprints Database: PREPRINT-BIORXIV Language: En Year: 2022 Document type: Preprint
Full text: 1 Collection: 09-preprints Database: PREPRINT-BIORXIV Language: En Year: 2022 Document type: Preprint