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Homologous and heterologous antibodies to coronavirus 229E, NL63, OC43, HKU1, SARS, MERS and SARS-CoV-2 antigens in an age stratified cross-sectional serosurvey in a large tertiary hospital in The Netherlands
Brenda M. Westerhuis; Erwin de Bruin; Felicity D Chandler; Chris R.B. Ramakers; Nisreen M.A. Okba; Wentao Li; Herman Goossens; Menno D de Jong; Berend Jan Bosch; Bart L Haagmans; Pieter LA Fraaij; Reina S Sikkema; Marion P.G. Koopmans.
Affiliation
  • Brenda M. Westerhuis; Viroscience, ErasmusMC
  • Erwin de Bruin; Viroscience, ErasmusMC
  • Felicity D Chandler; Viroscience, ErasmusMC
  • Chris R.B. Ramakers; Clinical chemistry, ErasmusMC
  • Nisreen M.A. Okba; Viroscience, ErasmusMC
  • Wentao Li; Biomolecular Health, University of Utrecht
  • Herman Goossens; Laboratory of Medical Microbiology, Uninversity of Antwerp
  • Menno D de Jong; Department of Medical Microbiology, Academic Medical Center
  • Berend Jan Bosch; Biomolecular Health, Utrecht University
  • Bart L Haagmans; Viroscience, ErasmusMC
  • Pieter LA Fraaij; Viroscience, ErasmusMC
  • Reina S Sikkema; Viroscience, ErasmusMC
  • Marion P.G. Koopmans; Viroscience, ErasmusMC
Preprint in En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20177857
ABSTRACT
Understanding the coronavirus (CoV) antibody landscape in relation to disease and susceptibility is critical for modelling of steps in the next phase during the current covid-19 pandemic. In March 2020, during the first month of the epidemic in The Netherlands, we performed cross sectional studies at two time points amongst patients of the Erasmus Medical Centre in Rotterdam, to assess the presence of antibodies against seasonal human coronaviruses (OC43, 229E, NL63, HKU1), emerging zoonotic coronaviruses (SARS, MERS) and SARS-CoV-2 in nine different age groups. We observed minimal SARS-CoV-2 reactivity early March (0.7% of sera), increasing to 3.0%, four weeks later, suggesting probably undetected cases during this early phase of the epidemic. Antibody responses were mostly coronavirus species specific at young age, but possible cross-reactivity between human seasonal CoVs was observed with increasing age.
License
cc_by_nc_nd
Full text: 1 Collection: 09-preprints Database: PREPRINT-MEDRXIV Type of study: Experimental_studies / Observational_studies / Rct Language: En Year: 2020 Document type: Preprint
Full text: 1 Collection: 09-preprints Database: PREPRINT-MEDRXIV Type of study: Experimental_studies / Observational_studies / Rct Language: En Year: 2020 Document type: Preprint