Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 315-326, 2020.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-1010537
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#Drug-resistance and metastasis are major reasons for the high mortality of ovarian cancer (OC) patients. Cyclooxygenase-2 (COX-2) plays a critical role in OC development. This study was designed to evaluate the effects of COX-2 on migration and cisplatin (cis-dichloro diammine platinum, CDDP) resistance of OC cells and explore its related mechanisms.@*METHODS@#Cell counting kit-8 (CCK-8) assay was used to detect the cytotoxicity effects of celecoxib (CXB) and CDDP on SKOV3 and ES2 cells. The effect of COX-2 on migration was evaluated via the healing test. Western blot and real-time quantitative polymerase chain reaction (qPCR) were used to analyze E-cadherin, vimentin, Snail, and Slug levels.@*RESULTS@#COX-2 promoted drug-resistance and cell migration. CXB inhibited these effects. The combination of CDDP and CXB increased tumor cell sensitivity, reduced the amount of CDDP required, and shortened treatment administration time. COX-2 upregulation increased the expression of Snail and Slug, resulting in E-cadherin expression downregulation and vimentin upregulation.@*CONCLUSIONS@#COX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Cell Movement
/
Polymerase Chain Reaction
/
Cisplatin
/
Drug Resistance, Neoplasm
/
Cell Line, Tumor
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Cyclooxygenase 2
/
Epithelial-Mesenchymal Transition
/
Celecoxib
Limits:
Female
/
Humans
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2020
Document type:
Article