hsa -let-7e-5p inhibits the migration of lung adenocarcinoma cells / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 1964-1970,1978, 2022.
Article
in Zh
| WPRIM
| ID: wpr-1038768
Responsible library:
WPRO
ABSTRACT
Objective @#To screen differentially expressed miRNAs and explore its effect and mechanism on cell mi- gration in lung adenocarcinoma (LUAD) .@*Methods @#Differentially expressed miRNAs in LUAD tissues and normal lung tissues were screened by miRNA microarrays,and then bioinformatics analysis was used to predict their poten- tial biological functions and signaling pathways.The cancer genome atlas (TCGA) analysis and quantitative real- time PCR (qRT-PCR) verified the expression level of hsa-let-7e-5p in LUAD tissues and cell lines.The effect of hsa-let-7e-5p cell migration in LUAD was examined by would healing experiment.After screening the underlying target genes by bioinformatics analysis ,the targeting relationship between hsa-let-7e-5p and DTX2,NME6, C8orf58,GATM and DHX57 were verified by qRT-PCR. @*Results @#The miRNA microarray results showed that 347 miRNAs were down-regulated while 229 miRNAs were up-regulated in lung adenocarcinoma tissues.Compared with normal lung tissue and cells,the expression level of hsa-let-7e-5p was significantly down-regulated.Besides,over- expression of hsa-let-7e-5p inhibitedLUAD cell migration.@*Conclusion @#Non-coding RNA hsa-let-7e-5p is down- regulated in LUAD and inhibits the migration of lung adenocarcinoma cells.DTX2,NME6,C8orf58,GATM and DHX57 are the potential target genes of hsa-let-7e-5p.
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Database:
WPRIM
Language:
Zh
Journal:
Acta Universitatis Medicinalis Anhui
Year:
2022
Document type:
Article