Establishing and identification of neurovascular unit in a tri-culture model using microfluidic chip / 中风与神经疾病杂志
Journal of Apoplexy and Nervous Diseases
; (12): 1070-1074, 2021.
Article
in Zh
| WPRIM
| ID: wpr-1039369
Responsible library:
WPRO
ABSTRACT
@#Objective We established a model by using microfluidic chip that is a horizontal side by side diffusion system through microporous separating three adjacent channels in a triple-culture using neurons,astrocytes,and bEnd.3.Methods (1)In the in vitro microphysiological system of neurovascular unit,we identified the seed density,seed time,seed sequence and seed method.(2)We adopt the repeated measures data of ANOVA to see whether there is significant difference in the viability when the three cell types are tri-cultured and separately mono-cultured.Results (1) In the novel in vitro NVU model,the seed density of the neurons,astrocytes and bEnd.3 is 5×10
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Database:
WPRIM
Language:
Zh
Journal:
Journal of Apoplexy and Nervous Diseases
Year:
2021
Document type:
Article