Enhancement of immune responses to hepatitis B DNA vaccine by superantigen SEA in mice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 681-685, 2005.
Article
in Zh
| WPRIM
| ID: wpr-237091
Responsible library:
WPRO
ABSTRACT
To investigate the adjuvant effect of plasmid DNA encoding superantigen SEA (D227A) (pmSEA) on immune responses induced by HBV DNA vaccine containing HBV preS2 and S antigen in BABL/c (H-2d). BALB/c mice were immunized intramuscular injection with HBV DNA vaccine (pHBVS2S) mixed with or without pmSEA plasmid. Antibodies againat HBV PreS2 and S antigen in the sera were accessed by Anti-HBs ELISA, and the HBsAg specific cytotoxic T lymphocytes (CTLs) activity was determined by 5 Chromium Release Assay. The HBs peptide-specific IFN-gamma secreting T cells were detected by ELISPOT. Anti-HBs antibody titers and CTLs activity in mice immunized with pmSEA + pHBVS2S group were significant higher (P < 0.05) than pHBVS2S DNA vaccine group. The ratio of IgG1/IgG2a (0.282) was apparently different from the group immunized with peptide (10). Mice immunized with HBV DNA vaccine plus adjuvant produce higher titer of IgG1 and IgG2a antibodies against HBV S antigen 1.36 and 1.73 time higher than that without adjuvant respectively. HBs peptide--specific IFN-gamma secreting T cells increased 2 - 3 times by the pmSEA adjuvant, compared to DNA vaccine group. HBV DNA vaccine (pHBVS2S) induces humoral and cellular immuno-responses in BALB/c mice, and the responses could be significantly boasted by the plasmid encoding mSEA. Therefore the pmSEA was a potential adjuvant for DNA vaccines.
Full text:
1
Database:
WPRIM
Main subject:
Staphylococcus aureus
/
Therapeutics
/
Blood
/
T-Lymphocytes, Cytotoxic
/
Adjuvants, Immunologic
/
Interferon-gamma
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Vaccination
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Hepatitis B Vaccines
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Superantigens
/
Vaccines, DNA
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2005
Document type:
Article