Effects of resveratrol on proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1878-1882, 2010.
Article
in Zh
| WPRIM
| ID: wpr-262235
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of resveratrol (Res) on in vitro proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes (RA FLS), and further to investigate the PI3 K/Akt/BAD signal mechanism.</p><p><b>METHOD</b>The inhibition rate of RA FLS was examined by MTT assay. Cell cycle and the amount of apoptotic cells was measured by flow cytometry. PI3K/Akt/BAD signal transduction proteins expression was measured by western blot.</p><p><b>RESULT</b>The living cells measured by MTT dose and time-dependently reduced in Res groups. In Res groups, the fraction of living cells in the S-phase and G2/M-phase decreased respectively, while that in G1-phase increased, the difference was statistically significant compared with the TNF-alpha group (P < 0.05). Flow cytometry demonstrated that the apoptosis rate increased with increased Res concentration. Res inhibited TNF-alpha induced phosphorylation of Akt and BAD in RA FLS.</p><p><b>CONCLUSION</b>Res can inhibit RA FLS proliferation and induce apoptosis through inhibition of PI3K/Akt/BAD signalling pathway. Res may provide a new therapeutic approach in treatment of RA.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Arthritis, Rheumatoid
/
Stilbenes
/
Synovial Membrane
/
Cell Cycle
/
Cells, Cultured
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Cell Biology
/
Cell Proliferation
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article