Effects of recombinant human interleukin-11 on LPS-induced intestinal epithelial cell injury in rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 529-533, 2014.
Article
in Zh
| WPRIM
| ID: wpr-269438
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of recombinant human interleukin-11 (rhIL-11) on the proliferation and apoptosis of rat intestinal epithelial cell line (IEC-6).</p><p><b>METHODS</b>IEC-6 cells were treated with LPS to establish necrotizing enterocolitis (NEC) model in vitro. rhIL-11 (100 ng/mL) was administered following LPS treatment and these cells were used as the IL-11 treatment group. The cells treated with normal saline only served as the control group. MTT assay was used to determine an optimal concentration (5-200 μg/mL) and time (1-24 h). MTT assay was used to measure the proliferation of IEC-6 cells at 3, 6, 9 and 12 hours after rhIL-11 treatment. Flow cytometry was used to evaluate the apoptosis of IEC-6 cells.</p><p><b>RESULTS</b>IEC-6 cells treated with various concentrations of LPS at various time points showed a lower proliferation than the control group (P<0.05). After 9 hours of rhIL-11 treatment, the proliferation activity of IEC-6 cells in the IL-11 treatment group significantly increased compared with the NEC model group without rhIL-11 treatment (P<0.05), reaching to the level of the control group. The total apoptotic and necrotic rate of IEC-6 cells in the IL-11 treatment group decreased significantly compared with the NEC model group without rhIL-11 treatment (P<0.01), but were still higher than the control group (P<0.05).</p><p><b>CONCLUSIONS</b>rhIL-11 can promote proliferation and reduce apoptotic and necrotic rates of IEC-6 cells treated with LPS.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Recombinant Proteins
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Cells, Cultured
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Lipopolysaccharides
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Interleukin-11
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Apoptosis
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Cell Proliferation
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Dose-Response Relationship, Drug
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Intestinal Mucosa
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2014
Document type:
Article