Enhancement of the bystander effect by tanshinone IIA in HSV-tK/GCV system is related to expression of connexin 43 mRNA / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 146-149, 2004.
Article
in Zh
| WPRIM
| ID: wpr-271032
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate enhancement of the bystander effect by tanshinone IIA (Tan) in HSV-tK/GCV system and the correlation with expression of connexin 43 mRNA.</p><p><b>METHODS</b>The cytotoxic effect in HSV-tK/GCV in cervical carcinoma cell line ME180 (ME) and ME/TK was examined by MTT assays. Cx43 mRNA expression was detected by fluor-quantitative RT-PCR.</p><p><b>RESULTS</b>Tan markedly increased sensitivity of ME/TK cells for GCV in HSV-tK/GCV system. In the presence of 2 micro g/ml GCV, compared with the absence of Tan (0 mol/L), an obvious decrease in survival rate was seen at any given mixture of ME and ME/TK cells exposed to 1.3 x 10(-9) mol/L Tan. Statistics showed significant difference (P < 0.05). However, enhancement of bystander mediated cell killing occurred only in the range of Tan concentrations used (1.3 x 10(-8), 1.3 x 10(-9) mol/L). RT-PCR showed that the ratio of relative copy number of Cx43 mRNA increased by 8.83 and 8.47-fold in ME cells exposed to 1.3 x 10(-8) and 1.3 x 10(-9) mol/L Tan, respectively.</p><p><b>CONCLUSION</b>For the first time we report that in cervical carcinoma ME180 cell line, Tan possesses a remarkable enhancing role on the bystander effect in the HSV-tK/GCV system. It is associated with up-regulation of Cx43 mRNA expression.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Phenanthrenes
/
Therapeutics
/
Thymidine Kinase
/
Genetic Therapy
/
Ganciclovir
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Cell Survival
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Uterine Cervical Neoplasms
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Simplexvirus
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Connexin 43
Limits:
Female
/
Humans
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2004
Document type:
Article