Effect of mesenteric lymphatic duct ligation on the system inflammation during the intestinal ischemia-reperfusion / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 469-471, 2008.
Article
in Zh
| WPRIM
| ID: wpr-273810
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To estimate the effect of the lymph duct ligation on systemic inflammatory factors and endotoxins during intestinal ischemia-reperfusion (I/R).</p><p><b>METHODS</b>Male SD rats underwent occlusion of superior mesenteric artery for 60 min followed by reperfusion for 120 min plus lymph duct ligation or not. Forty rats were randomly divided into 4 groups: group A (blank); group B (sham); group C (intestinal I/R); group D (intestinal I/R plus lymph duct ligation). Mesenteric lymph nodes were harvested for standard bacteriologic cultures. The endotoxin, D-lactate, diamine oxidase (DAO), and cytokines in serum were detected.</p><p><b>RESULTS</b>The rates of bacterial translocation to mesenteric lymph nodes were 40% in group C and 20% in group D. No positive lymph node cultures were encountered in any of group A and B. The serum cytokines (except for sICAM-1) , D-lactate, DAO and endotoxin levels were lower in group D than those in group C (P<0.05), but both were higher than those in group A and B (P<0.05).</p><p><b>CONCLUSION</b>During intestinal I/R injury, blockage the lymph flow from gut into bloodstream decreases the levels of cytokines, and significantly attenuates the increase in intestinal permeability.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
General Surgery
/
Reperfusion Injury
/
Rats, Sprague-Dawley
/
Disease Models, Animal
/
Inflammation
/
Intestinal Diseases
/
Intestines
/
Ligation
/
Lymph Nodes
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2008
Document type:
Article