Determination and comparison of plasma protein binding rate of alkaloids from seed of Strychnou nux-vomica / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 185-188, 2011.
Article
in Zh
| WPRIM
| ID: wpr-289402
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the plasma protein binding rates of brucine and strychnine in total alkaloids from the seed of Strychnou nux-vomica, and make comparison with the single components at the same concentration.</p><p><b>METHOD</b>Ultrafiltration was employed to determine the rat the plasma protein binding rate of the alkaloids from the seed of S. nux-vomica. The plasma concentrations were measured by RP-HPLC.</p><p><b>RESULT</b>The protein binding rates of brucine were (65.60 3.01)%, (68.20 +/- 7.80)%, (59.58 +/- 3.78)% when the plasma concentrations was 0.520, 1.300, 2.600 mg x L(-1), respectively. The protein binding rates of strychnine was (66.17 +/- 6.36)%, (67.10 +/- 2.52)%, (57.21 +/- 0.79)% when the plasma concentrations were 0.936, 2.340, 4.680 mg x L(-1) respectively. As to the total alkaloids from the seed of S. nux-vomica, The protein binding rate of brucine was (62.19 +/- 2.45)%, (69.55 +/- 5.84)%, (61.76 +/- 3.68)% when the plasma concentrations were 0.519, 1.288, 2.607 mg x L(-1), respectively. And the protein binding rates of strychnine were (54.79 +/- 3.55)%, (57.13 +/- 4.49)%, (59.31 +/- 3.65)% when the plasma concentrations were 0.940, 2.338, 4.674 mg x L(-1), respectively.</p><p><b>CONCLUSION</b>Brucine and strychnine have medium capacity in binding to plasma protein. In comparison with the single component of the same concentration, the protein binding rate of brucine in total alkaloids shows little difference, while there seems to be an obvious decrease for strychnine.</p>
Full text:
1
Database:
WPRIM
Main subject:
Protein Binding
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Seeds
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Drugs, Chinese Herbal
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Pharmacokinetics
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Blood Proteins
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Chemistry
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Rats, Sprague-Dawley
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Strychnos
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Alkaloids
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Document type:
Article