Pinching spine: A potential treatment for depression / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 272-279, 2014.
Article
in En
| WPRIM
| ID: wpr-289680
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether pinching spine (PS, i.e. , a traditional Chinese manipulative therapy) is beneficial to ameliorating the depressive state (including behavioral deficit, retardative weight gain and decreased sucrose consumption) in a rat model of depression induced by chronic unpredictable stress (CUS) and to explore the candidate mechanism of action.</p><p><b>METHODS</b>PS was performed on rats' spine once daily for 1 week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were applied on different dates: before stress (d0), at the end of stress (d21) and after PS treatment (d28), respectively. Then the rats' hippocampuses were performed genome-wide microarray analysis, and the expression levels of several genes were evaluated by real-time polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Exposure to CUS resulted in decreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment. The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., were down-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restriction of activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatment significantly inverted these changes. Furthermore, increase or decrease in gene expression evaluated by realtime PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis.</p><p><b>CONCLUSION</b>PS showed a potential antidepressant-like effect, of which the action mechanism might be due to gene expression regulation in hippocampus.</p>
Key words
Full text:
1
Database:
WPRIM
Main subject:
Spine
/
Therapeutics
/
Rats, Sprague-Dawley
/
Musculoskeletal Manipulations
/
Depression
/
Medicine, Chinese Traditional
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Chinese journal of integrative medicine
Year:
2014
Document type:
Article