DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1690-1696, 2009.
Article
in Zh
| WPRIM
| ID: wpr-296871
Responsible library:
WPRO
ABSTRACT
External Guide Sequences (EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression. They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus (HCMV). The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target mRNA sequence in vitro. A reduction of more than 50% in the levels of UL49 expression was observed in human cells treated with the DNA-based EGS1386 targeted UL49 assayed by fluorescent quantization PCR and Western blotting. This results showed that the DNA-EGS1386 can effectively guide the RNase P cut the target mRNA. Therefore, DNA-EGS can develop into a new gene silencing technology and potential of the anti-viral reagents.
Full text:
1
Database:
WPRIM
Main subject:
Oligodeoxyribonucleotides
/
Pharmacology
/
Virology
/
DNA, Viral
/
RNA, Messenger
/
Base Sequence
/
Gene Expression Regulation, Viral
/
Viral Structural Proteins
/
Chemistry
/
Cytomegalovirus Infections
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2009
Document type:
Article