Study on preparation and quantity determination of Scrophularia ningpoensis dispensing granules / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop the preparation method and to determine the quantity of effective constituents of Scrophularia ningpoensis dispensing granules.</p><p><b>METHOD</b>Nine experiments were carried out through L9 (3(4)) orthogonal design. The contents of harpagoside and cinnamic acid were determined by HPLC method.</p><p><b>RESULT</b>The optimal extraction process was as follows: the slices were soaked in water in the ratio of 1:8 for 0.5 h, then they were decocted for 2 h. For the second time, 6 times of water was added and sustained for 1.5 h. Harpagoside and cinnamic acid were linear within the ranges of 0.0776-1.552 microg (r=0.9999) and 0.01446-0.4339 microg (r=0.9999), respectively. The average recoveries were 100.4% (RSD 1.98%, n=6) and 98.27% (RSD1.35%, n=6), respectively. Ten batches of granules were determined.</p><p><b>CONCLUSION</b>The extraction process is scientific and the determination method is suitable for quality control of Scrophularia ningpoensis dispensing granules.</p>