Establishment of a high-resolution 2-D reference map of human spermatozoal proteins from 12 fertile sperm-bank donors / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 321-329, 2007.
Article
in En
| WPRIM
| ID: wpr-310507
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To extend the analysis of the proteome of human spermatozoa and establish a 2-D gel electrophoresis (2-DE) reference map of human spermatozoal proteins in a pH range of 3.5-9.0.</p><p><b>METHODS</b>In order to reveal more protein spots, immobilized pH gradient strips (24 cm) of broad range of pH 3-10 and the narrower range of pH 6-9, as well as different overlapping narrow range pH immobilized pH gradient (IPG) strips, including 3.5-4.5, 4.0-5.0, 4.5-5.5, 5.0-6.0 and 5.5-6.7, were used. After 2-DE, several visually identical spots between the different pH range 2-D gel pairs were cut from the gels and confirmed by mass spectrometry and used as landmarks for computer analysis.</p><p><b>RESULTS</b>The 2-D reference map with pH value from 3.5 to 9.0 was synthesized by using the ImageMaster analysis software. The overlapping spots were excluded, so that every spot was counted only once. A total of 3872 different protein spots were identified from the reference map, an approximately 3-fold increase compared to the broad range pH 3-10 IPG strip (1306 spots).</p><p><b>CONCLUSION</b>The present 2-D pattern is a high resolution 2-D reference map for human fertile spermatozoal protein spots. A comprehensive knowledge of the protein composition of human spermatozoa is very meaningful in studying dysregulation of male fertility.</p>
Full text:
1
Database:
WPRIM
Main subject:
Physiology
/
Reference Values
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Semen
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Spermatozoa
/
Tissue Donors
/
Sperm Banks
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Proteins
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Chemistry
/
Proteome
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Spectrometry, Mass, Electrospray Ionization
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
Asian Journal of Andrology
Year:
2007
Document type:
Article