Construction of RegIα over-expression vector and its effect on proliferation and apoptosis of gastric cancer cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 499-505, 2010.
Article
in Zh
| WPRIM
| ID: wpr-319869
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct RegIα over-expression vector and to evaluate the effect of RegIα on the proliferation and apoptosis of gastric cancer MKN28 cells in vitro.</p><p><b>METHODS</b>Full sequence of RegIα cDNA was amplified from normal gastric tissue samples by RT-PCR and cloned into pIRES2-EGFP vector. RT-PCR and Western blot were performed to detect expression levels of RegIα in MKN28 cells. The effects of over-expression RegIα on cell proliferation was measured by MTT assay and apoptosis was detected by flow cytometry.</p><p><b>RESULT</b>RegIα cDNA over-expression vector of pIRES2-RegIα-EGFP was successfully constructed. The expressions of RegIα in MKN28 cells, including mRNA and protein levels, were significantly increased after stable transfection, which resulted in cell proliferation and anti-apoptotic effect induced by H(2)O(2).</p><p><b>CONCLUSION</b>The over-expression of RegIα can promote cell proliferation and reduce cell apoptosis when induced by H(2)O(2) in gastric cancer cells.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Plasmids
/
Stomach Neoplasms
/
Transfection
/
Apoptosis
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Cell Line, Tumor
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Cell Proliferation
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Document type:
Article