Experimental study on anti-tumor effects of cortex Acanthopanacis senticosus in vivo and in vitro / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 55-58, 2004.
Article
in Zh
| WPRIM
| ID: wpr-320251
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To provide a basis for development and preparation of the new anti-tumor agents from Cortex A-canthopanacis senticosus (CAS), through isolating the active substances from CAS and studying the anti-tumor effect of CAS extracts in vivo and in vitro.</p><p><b>METHODS</b>The effects of CAS extracts and its isolated ingredients on tumor cell proliferation in vitro was determined by 3H-TdR incorporation; the anti-tumor component of CAS was isolated and purified by chromatography; the tumor bearing mice model was established by injecting tumor cell subcutaneously, and the model was used to observe the anti-tumor effect of CAS extract administered through gastrogavage.</p><p><b>RESULTS</b>CAS extract showed obvious inhibition on tumor cell proliferation originated from multiple tissues (P < 0.01) and displayed a better dose-effect relationship. After orally taken CAS extract, the general condition of mice in the experimental group were better than that in the untreated control group, revealing a slower growth and significantly prolonged survival period (P < 0.01). A protein component, having inhibitory effect on tumor cell proliferation and the molecular weight was 64 kda, it was isolated by the thin layer gel chromatography.</p><p><b>CONCLUSION</b>CAS has not only the in vitro inhibitory effect on cell proliferation of multiple kinds of tumor, but also a good anti-tumor effect in vivo. The anti-tumor activity of CAS is correlated with a protein component with the molecular weight of 64 kda. Further isolation, purification, study on mechanism will provide scientific evidence for clinical application and development of CAS in anti-tumor effect.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Drugs, Chinese Herbal
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Tumor Cells, Cultured
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Leukemia, T-Cell
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Cell Division
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HL-60 Cells
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Eleutherococcus
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Dose-Response Relationship, Drug
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Mice, Inbred BALB C
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2004
Document type:
Article
Publication country:
CHINA
/
CN
/
REPUBLIC OF CHINA