Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1838-1841, 2008.
Article
in Zh
| WPRIM
| ID: wpr-321805
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.</p><p><b>METHODS</b>The inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis. The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.</p><p><b>RESULTS</b>The IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 cells was 11.03 nmol/L and 8.81 micromol/L, respectively. Rapamycin alone caused cell cycle arrest of the Hep-2 cells in G1 phase. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hep-2 cells (q > 1.15, King's Formula), causing significantly increased apoptosis ratio and growth inhibition rate of Hep-2 cells.</p><p><b>CONCLUSION</b>Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hep-2 cells.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Tumor Cells, Cultured
/
Carcinoma, Squamous Cell
/
Laryngeal Neoplasms
/
Cisplatin
/
Apoptosis
/
Sirolimus
/
Cell Proliferation
/
Drug Synergism
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2008
Document type:
Article
Country of publication:
CHINA
/
CN
/
REPUBLIC OF CHINA