Construction of MHV-A59 damp-heat mouse model and analysis of the relevant indices / 南方医科大学学报
Journal of Southern Medical University
; (12): 2452-2454, 2010.
Article
in Zh
| WPRIM
| ID: wpr-323637
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the impact of inflammation, water metabolism and immune function on the establishment of a mouse model of damp-heat syndrome with MHV-A59 infection.</p><p><b>METHODS</b>Twenty-four mice were randomly divided into control group, virus group, damp-heat group and model group. The peripheral blood CD4(+) and CD8(+) lymphocytes were detected by flow cytometry, and the serum levels of IFN-γ and IL-4 were assayed by ELISA. The expressions of NF-κB and AQP4 in the liver and stomach were determined using immunohistochemistry.</p><p><b>RESULTS</b>The expression of NF-κB and CD4(+)/CD8(+) ratio in the virus and model groups were significantly higher than those in the damp-heat and control groups, while the expression of AQP4 was significantly higher in the model and damp-heat groups than in the other groups. Compared with the control group, the model group showed a significantly higher ratio of IFN-γ/IL-4.</p><p><b>CONCLUSIONS</b>MHV-A59 virus is the main cause of elevated NF-κB expression and CD4(+)/CD8(+)/ ratio, while damp-heat syndrome is responsible for increased AQP4 expression, and their synergistic effect results in increased IFN-γ/IL-4 ratio. The mouse model established using MHV-A59 virus and the damp-heat factors can mimic damp-heat syndrome described in traditional Chinese medicine theory.</p>
Full text:
1
Database:
WPRIM
Main subject:
Virology
/
Blood
/
Interleukin-4
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Interferon-gamma
/
CD4-CD8 Ratio
/
Murine hepatitis virus
/
Diagnosis
/
Disease Models, Animal
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Aquaporin 4
/
NF-kappa B p50 Subunit
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2010
Document type:
Article