Research on an improved Lowry method to determine content of protein in Sabin IPV / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 495-497, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332455
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the content determination of protein in Sabin IPV.</p><p><b>METHODS</b>Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.</p><p><b>RESULTS</b>The method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.</p><p><b>CONCLUSION</b>The method can be used to determine the micro content of protein in vaccines.</p>
Full text:
1
Database:
WPRIM
Main subject:
Phenols
/
Trichloroacetic Acid
/
Calibration
/
Poliovirus Vaccine, Oral
/
Proteins
/
Chemistry
/
Chemistry Techniques, Analytical
/
Amino Acids
/
Metabolism
/
Methods
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Document type:
Article