One-step multiplex RT-PCR for identifying common fusion transcripts in childhood acute lymphoblastic leukemia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 332-336, 2015.
Article
in Zh
| WPRIM
| ID: wpr-346153
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficiency of one-step multiplex RT-PCR for identifying four common fusion transcripts (TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL) in children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Total RNA was extracted from bone marrow samples of 76 children who were newly diagnosed with ALL between January 2003 and December 2010. These RNAs were analyzed for TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL by one-step multiplex RT-PCR or common nested-multiplex PCR. The PCR products were confirmed by DNA sequencing.</p><p><b>RESULTS</b>TEL/AML1 was found in 12 cases (the length of products was 298 bp in 9 cases and 259 bp in 3 cases), E2A/PBX1 was found in 3 cases (the length of products was 373 bp), BCR/ABL was found in 1 case (the length of products was 2 124 bp), and MLL/AF4 was found in 7 cases (the length of products was 427 bp in 1 case and 673 bp in 6 cases) using one-step multiplex RT-PCR combined with DNA sequencing. The results were consistent with those using common nested-multiplex PCR.</p><p><b>CONCLUSIONS</b>One-step multiplex RT-PCR may be another alternative for detection of common fusion transcripts in children with ALL.</p>
Full text:
1
Database:
WPRIM
Main subject:
Oncogene Proteins, Fusion
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Fusion Proteins, bcr-abl
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Sequence Analysis, DNA
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Reverse Transcriptase Polymerase Chain Reaction
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Myeloid-Lymphoid Leukemia Protein
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Core Binding Factor Alpha 2 Subunit
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Multiplex Polymerase Chain Reaction
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Genetics
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Methods
Type of study:
Prognostic_studies
Limits:
Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2015
Document type:
Article