Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants / 生物工程学报
Chinese Journal of Biotechnology
; (12): 461-467, 2011.
Article
in Zh
| WPRIM
| ID: wpr-351512
Responsible library:
WPRO
ABSTRACT
To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the comparison with the wild strain. The fermentation in a medium composed of yeast extract (10 g/L), peptone (10 g/L) and D-glucose (20 g/L) resulted in the lipid yield of the mutant (C4) with 4.07% (W/W) compared with that of the wild strain (1.87%).
Full text:
1
Database:
WPRIM
Main subject:
Peptones
/
Pharmacology
/
Atmosphere
/
Temperature
/
Yeasts
/
Culture Media
/
Biofuels
/
Fermentation
/
Genetics
/
Glucose
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Document type:
Article