Role of IL-12 in lung ischemia-reperfusion injury in rats / 中华麻醉学杂志

ABSTRACT

Objective To investigate the role of IL-12 in lung ischemia-reperfusion(L/R)injury in rats.Methods Twenty-four healthy male SD rats,8-10 weeks,weighing 250-280 g,were randomly divided into 3 groups(n =8 each):sham operation group(S),I/R group,IL-12 monoclonal antibody group(group IL-12).LungI/R was induced by clamping the left hilum of lung for 60 min followed by 120 min reperfusion in groupsI/R and IL-12.IL-12 monoclonal antibody 200tg/kg was injected via the caudal vein at I h before clamping the left hilum.The blood samples were collected at the end of reperfusion,the rats were then sacrificed and lungs removed for determination of the plasma TNF-α concentration,Th1 and Th2 levels,W/D lung weight ratio,MDA content,MPO activity,PaO2 and PaCO2 in lung tissues and for microscopic examination.Results Compared with group S,W/D ratio and the level of MPO,MDA and TNF-α were significantly increased in groups I/R and IL-12,and PaO2 was significantly decreased,and PaCO2,Th1 level and Th1/Th2 sere significantly increased in group I/R(P ＜0.01),and no significant change was found in PaO2,PaCO2,the level of Th1 and Th2,and Th1/Th2 in group IL-12(P ＞ 0.05).PaCO2,W/D ratio,the levels of MDA,MPO,plasma TNF-u,Th1 and Th1/Th2 were significantly lower,and Th2 level and PaCO2 were significantly higher in group IL-12 than in group I/R(P ＜ 0.01).The pathologic changes of the lung were attenuated in group IL.12.Conclusion IL-12 is involved in the lung I/R injury through inducing Th1/Th2 imbalance in rats.