Loss of β-catenin inhibits Stat-5α phosphorylation in bcr-abl induced leukemia cells / 白血病·淋巴瘤
Journal of Leukemia & Lymphoma
; (12): 593-595, 2010.
Article
in Zh
| WPRIM
| ID: wpr-472211
Responsible library:
WPRO
ABSTRACT
Objective To investigate the influence of β-catenin gene deletion on Stat-5α phosphorylation in bcr-abl induced leukemia cells. Methods The established conditonal hematopoitic β-catenin knockout mice were used to isolate bone marrow cells. Exogenous bcr-abl fusion gene was transduced to these bone marrow cells by retroviral infection with intent to transfom them to leukemia cells.Immunofluorescence was performed to detect the phosphorylation status of Stat-5α in both β-catenin deletion cells and control cells. bcr-abl transcription and protein levels were evaluated with real-time PCR and western blotting. Results Phosphorylation of Stat-5α was reduced significantly in β-catenin deletion leukemia cells on comparison with control cells despite that total Stat-5α protein showed no obvious changes. Total tyrosine phosphorylation and bcr-abl protein expression were reduced in bcr-abl induced β-catenin deletion CML cells,on the contrary, both of the reduction were not seen in bcr-abl induced β-catenin deletion ALL cells.Conclusion Loss of β-catenin inhibits both Stat-5α phosphorylationin and bcr-abl expression in bcr-abl induced leukemia cells.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Leukemia & Lymphoma
Year:
2010
Document type:
Article