Electrocorticogram and behavior of electrically kindled epileptic rats / 中国比较医学杂志

ABSTRACT

Objective The current study was aimed to establish an electrically amygdala kindling model of refractory epilepsy in Sprague-Dawley rats, and to study their changes of electrocorticagram ( ECoG ) .Materials and methods Male Sprague-Dawley rats were used in the experiment.Two-polar electrode was implanted into the right amygdala stereotactically.Two weeks after the surgery, electrical stimulations were given to elicit the grade 4 seizure in all animals with the fast kindling protocol.Rekindling was administrated after the first kindling.The after discharge threshold ( ADT) and the number of stimulus were reassessed in the two kindling protocols.ECoG and the behavior of the animals were recorded during the whole experiment.Result The changes of ECoG and behavior: Animals showed stage 1 -3 seizure when the ADT was assessed.While during the kindling period, the animals showed generalized convulsion with stage 4-5 seizure.ECoG showed sharp waves, spike waves, sharp-slow waves and spike-slow waves.Statistical analysis showed that the ADT was not significantly increased at two weeks after kindling ( from 83.33 ±22.29μA to 84.17 ±16.76μA, P=0.923 ) .The number of stimulus given to elicit the stage 4 convulsion was not significantly increased as well ( from 4.41 ±2.27 to 5.58 ±3.96, P=0.231 ) .Conclusions Rapid kindling model of epilepsy in rats is an effective epilepsy model, which is stable for 2 weeks.