Uncertainty Evaluation of the Determination of Lamotrigine in Human Plasma by LC-MS/MS / 中国药师
China Pharmacist
; (12): 854-858, 2016.
Article
in Zh
| WPRIM
| ID: wpr-493918
Responsible library:
WPRO
ABSTRACT
Objective:To evaluate the uncertainty in the determination of lamotrigine(LTG)in human plasma by LC-MS/ MS. Methods:The uncertainty sources in the determination of LTG were analyzed and the uncertainty was evaluated and combined. Re-sults:The expanded uncertainty of LTG at low(0. 050 4 μg· ml - 1 )and high(1. 27 μg· ml - 1 )concentrations was 0. 005 18 μg· ml - 1 and 0. 066 4 μg· ml - 1 ,respectively(P = 95% ,k = 2). Conclusion:The uncertainty in the determination of LTG in human plasma by LC-MS/ MS is mainly caused by the protein precipitation recovery,matrix effect and sample preparation at low concentration, and by the matrix effect,sample preparation and repeatability at high concentration.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2016
Document type:
Article