Simultaneous Determination of Four Glycosides in Microctis Folium by UPLC / 中国药师
China Pharmacist
; (12): 1079-1081, 2016.
Article
in Zh
| WPRIM
| ID: wpr-494929
Responsible library:
WPRO
ABSTRACT
Objective:
To develop a UPLC method for determining four glycosides in microctis folium.Methods:
The UPLC deter-mination was performed on an Agilent SB -C18 (3.0 mm ×50 mm, 1.8 μm) colume with 0.05% acetonitrile -phosphate solution as the mobile phase.The detection wavelength was set at 320 nm.The flow rate was 0.3 ml· min-1.The column temperature was 25℃.Results:
Narcissoside, isovitexin, kaempferol-3-rutinoside and isorhamnetin-3-O-glucoside all showed good linearity with the re-covery of 98.89%,99.03%, 97.00%and 97.41%, and RSD of 0.41%, 0.84%,0.44%and 0.80%, respectively (n=9).Con-clusionThe method is convenient with good stability , repeatability and sample recovery rate , which provides basis for the quality eval-uation and control of microctis folium.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2016
Document type:
Article
Publication country:
CHINA
/
CN
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REPUBLIC OF CHINA