A study on the influential factors of G6PD/6PGD specific value assay in the heterozygotes of G6PD gene variants in female patients / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-528053
Responsible library:
WPRO
ABSTRACT
Objective To improve the detection of G6PD heterozygotes in female patients by the optimum experimental factors of G6PD/6PGD specific value assay.Methods (1)Identifying the mutations of G6PD gene with the use of amplified refractory mutation system (ARMS).(2)Measuring the G6PD/6PGD specific value.Results According to the data analysed by statistics and ROC curve, the optimum experimental factors included that the incubation temperature was 37℃,the substrate concentrations were 0.78 mmol/L G6PNa2 and 0.195mmol/L NADP+, the reaction time was 10min.Conclusion The optimum experimental factors of G6PD/6PGD specific value assay may be used to improve the detection rate of G6PD heterozygotes in female patients.
Full text:
1
Database:
WPRIM
Type of study:
Prognostic_studies
Language:
Zh
Journal:
International Journal of Laboratory Medicine
Year:
2006
Document type:
Article