Determination of Oridonin in Rabdosia nervosa by HPLC / 中药新药与临床药理
Article
in Zh
| WPRIM
| ID: wpr-575802
Responsible library:
WPRO
ABSTRACT
Objective To develop a HPLC method for the determination of oridonin in the leaves and stems of Rabdosia nervosa.Methods All of reference substances and samples were separated with the mobile phase of methanol:water (45 ∶55) under isocratic elution for 20 min on a Kromasil C18 reversed-phase column(250 mm?4.0 mm,5 ?m),the flow rate was 1.0 mL?min-1,the detection wavelength was 235 nm,and the column temperature was 30 ℃.Results The content of oridonin was 0.047 mg/g in stems and 0.791 mg/g in leaves,the content in stems being about one seventeenth of that in leaves.The average recovery of oridonin was 98.33 %.Conclusion This method is simple,sensitive,accurate and suitable for quantitative determination of oridonin in Rabdosia nervosa.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
2000
Document type:
Article