?-Synuclein phosphorylation takes part in mouse dopaminergic neuron protection / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-577657
Responsible library:
WPRO
ABSTRACT
Objective To study the role of phosphorylation at Serine 129 in regulating the neurotoxicity of ?-synuclein. Methods Wild type and phosphomimic mutant ?-synuclein genes were over-expressed in mouse dopaminergic cells MN9D using retrovirus. The cell viability was examined using CCK-8 assay and cell morphology was observed by immunofluorescence microscopy. Results The result of real time PCR showed that WT/?-SYN and S129D/?-SYN genes were overexpressed in MN9D as compared to uninfected MN9D and vector control group(P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2006
Document type:
Article