Determination of the Content of Caffeic Acid in Chanhou Tieshijin by HPLC / 中国中医药信息杂志
Article
in Zh
| WPRIM
| ID: wpr-578835
Responsible library:
WPRO
ABSTRACT
Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm?4.6 mm, 5 ?m). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 ?L. Results The linear range of caffeic acid was 0.052~0.26 ?g (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Document type:
Article