Inhibition to hepatoma cells by anti-sense RNA targeting the key site of hTERT gene / 基础医学与临床

ABSTRACT

Objective To study the inhibition effects on hepatoma cells growth by the anti-sense RNA targeting C-MYC binding site on regulation region of hTERT promoter.Methods The rAd virus which express anti-sense RNA complementary to the C-MYC binding site on regulation region of hTERT were constructed using the method of homologous recombination in bacteria cells.The apoptosis of HepG2.2.15 cells infected by rAd-asmycb was detected by the method of Annexin V-FITC/PI labeling,and the morphological changes were observed by electronic microscopy.TRAP-PCR-ELISA and RT-PCR were used to detecte the relative telomerase activity(RTA) and gene transcription at mRNA level of hTERT.Results Cell growth of HepG2.2.15 was retarded and about 40.7% tumor cells were lead to apoptosis.RTA of anti-sense RNA treated cells(1.175) was much lower than the control cells(4.200,P