Pseudolaric acid B inhibits growth of human gastric carcinoma cells in vitro / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-591603
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of pseudolaric acid B(PLAB) on growth of human gastric carcinoma cells in vitro.Methods The expression of PPAR? was detected by RT-PCR;the effect of PLAB on cell growth was tested by MTT;Hoechst33342/PI and DNA gel electrolysis were employed to examine apoptosis;cell cycle was checked by flow cytometry.Results When treated with 0.1~10 ?mmol/L PLAB for 72,the proliferation of MGC803 cells was significantly inhibited.The proportion of MGC803 cells at G2 phase was significantly increased when treated with 10 ?mmol/L PLAB after 48 h,and showed an apparent G2 phase arrest.After treatement with PLAB for 72,typical apoptotic changes were observed.The expression of PPAR? was at a low level in MGC803 cells and up-regulated when treated with 10 ?mmol/L PLAB for 48 h(P
Full text:
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Database:
WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2006
Document type:
Article