Content Determination of Astragaloside Ⅳ in Qingshen Jianfei Tablets by HPLC-ELSD / 中国中医药信息杂志
Article
in Zh
| WPRIM
| ID: wpr-683618
Responsible library:
WPRO
ABSTRACT
Objective To establish a HPLC-ELSD method for determining the content of astragaloside Ⅳ in Qingshen Jianfei Tablets. Methods Stationary phase was C_(18) column (4.6 mm?250 mm, 5 ?m), mobile phase was acetonitrice-water (35 : 65). The evaporation temperature was 120 ℃, the pulverization temperatire was 80 ℃, the flow rate was 1.6 mL/min and column temperature was 35 ℃. Results The standard curve was linear within the range of 1.03~8.24 ?g, r=0.9999. The average recovery was 99.35% with RSD = 1.36% (n = 6). Conclusion The established method is simple and accurate, with good reproducibility and high precision, and suitable for the determination of astragaloside Ⅳ in Qingshen Jianfei Tablets.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Document type:
Article