Effects of three kinds of liquid media on the biofilm formation of Staphylococcus epidermidis / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
; (12): 245-247, 2018.
Article
in Zh
| WPRIM
| ID: wpr-694829
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effects of 3 kinds of broth media,including tryptose soya broth(TSB),LB and M-H,on the biofilm formation of Staphylococcus epidermidis (S.epidermidis).Methods The effects of TSB,LB and M-H broth media on the biofilm formation of S.epidermidis were investigated by the construction of bacterial biofilm in 96-well and 6-well microplates and the crystal violet straining for the semi-quantitative analysis and microscopic observation of the bacterial biofilm.The effects of TSB,LB and M-H broth media on the expression of adhesion-related genes in S.epidermidis were determined by the extraction of bacterial total RNA,reverse transcription and real-time PCR.Results Compared with LB (0.149 ± 0.047) and M-H (0.323 ± 0.003) media,TSB medium (2.954 ± 0.287) could significantly promote the biofilm formation of S.epidermidis (TSB vs LB,t =16.706,P < 0.01;TSB vs M-H,t =15.877,P < 0.01).Compared with LB medium,TSB medium could significantly enhance the expression of icaA gene (t =9.667,P<0.01) but inhibit icaR gene (t =13.283,P<0.01).Conclusion Compared with LB and M-H broth media,TSB medium may significantly improve the biofilm formation and the expression of adhesion-related gene icaA of S.epidermidis.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Clinical Laboratory Science
Year:
2018
Document type:
Article